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Sequentially aerated membrane biofilm reactors for autotrophic nitrogen removal: microbial community composition and dynamics.

ABSTRACT: Membrane-aerated biofilm reactors performing autotrophic nitrogen removal can be successfully applied to treat concentrated nitrogen streams. However, their process performance is seriously hampered by the growth of nitrite oxidizing bacteria (NOB). In this work we document how sequential aeration can bring the rapid and long-term suppression of NOB and the onset of the activity of anaerobic ammonium oxidizing bacteria (AnAOB). Real-time quantitative polymerase chain reaction analyses confirmed that such shift in performance was mirrored by a change in population densities, with a very drastic reduction of the NOB Nitrospira and Nitrobacter and a 10-fold increase in AnAOB numbers. The study of biofilm sections with relevant 16S rRNA fluorescent probes revealed strongly stratified biofilm structures fostering aerobic ammonium oxidizing bacteria (AOB) in biofilm areas close to the membrane surface (rich in oxygen) and AnAOB in regions neighbouring the liquid phase. Both communities were separated by a transition region potentially populated by denitrifying heterotrophic bacteria. AOB and AnAOB bacterial groups were more abundant and diverse than NOB, and dominated by the r-strategists Nitrosomonas europaea and Ca. Brocadia anammoxidans, respectively. Taken together, the present work presents tools to better engineer, monitor and control the microbial communities that support robust, sustainable and efficient nitrogen removal.

SUBMITTER: Pellicer-Nacher C

PROVIDER: S-EPMC3896934 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature

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Sequentially aerated membrane biofilm reactors for autotrophic nitrogen removal: microbial community composition and dynamics.

Pellicer-Nàcher Carles C Pellicer-Nàcher Carles C Franck Stéphanie S Gülay Arda A Ruscalleda Maël M Terada Akihiko A Al-Soud Waleed Abu WA Hansen Martin Asser MA Sørensen Søren J SJ Smets Barth F BF

Microbial biotechnology 20131001 1

Membrane-aerated biofilm reactors performing autotrophic nitrogen removal can be successfully applied to treat concentrated nitrogen streams. However, their process performance is seriously hampered by the growth of nitrite oxidizing bacteria (NOB). In this work we document how sequential aeration can bring the rapid and long-term suppression of NOB and the onset of the activity of anaerobic ammonium oxidizing bacteria (AnAOB). Real-time quantitative polymerase chain reaction analyses confirmed ...[more]

PMID: 24112350

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Project description:This study investigated the potential of Membrane-Aerated Biofilm Reactors (MABRs) for mainstream nitrogen removal via partial nitration/anaerobic ammonium oxidation (anammox). Four laboratory-scale MABRs were operated with real municipal wastewater characterized by low concentrations of nitrogen (varying between 31 and 120 mg-NH4-N L-1) and the presence of biodegradable organic carbon (soluble COD (sCOD) between 7 and 230 mg-O2 L-1). Two reactors were operated with different aeration strategies (intermittent vs. continuous), the other two with differences in biomass retention (recirculation or removal of detached biomass). Keeping a constant HRT caused instabilities due to difficulties with setting the optimal oxygen flux for the respective surface loadings (1.6-6 g-NH4-N m-2 d-1). Operating the MABRs with a constant surface loading (2 g-NH4-N m-2 d-1) resulted in higher and more stable total nitrogen (TN) removal independent of the aeration strategy. The intermittently aerated MABR improved from an average TN removal of 23%-69%, the continuously aerated MABR from 20% to 50% TN removal. Independent of the feeding strategy, the continuously aerated reactor removed slightly more ammonium (80-95%) compared to the intermittently aerated reactor (74-93%). Limiting the oxygen supply by intermittent aeration proofed successful to favor partial nitritation and anammox. Continuous aeration did not achieve stable suppression of nitrite oxidizing bacteria (NOB). Of the removed ammonium, approx. 26% were left in the effluent as nitrate (only 10% with intermittent aeration). Recirculation of the detached biomass resulted in reattachment onto the biofilm or membrane surface. This recirculation led to significantly higher biomass retention times and thus to better performance. Removing detached biofilm from the reactor caused a slightly lower TN removal of 33% compared to 45% with reattachment, while average ammonium removal was 58% compared to 63%, respectively. Scouring events had a significant impact on the overall operation, resulting in short term losses of TN removal capacities of 50-100%. The microbial community composition was different depending on the aeration strategy and biomass retention. The continuously aerated reactor contained significantly more AOB than the intermittently aerated MABR. The reactor with biomass retention contained less ammonium oxidizing bacteria (AOB), compared to the reactor with low biomass retention. In all MABRs, anammox bacteria established in the biofilm after an initial drop in abundance.

Dataset Information

Sequentially aerated membrane biofilm reactors for autotrophic nitrogen removal: microbial community composition and dynamics.

Publications

Sequentially aerated membrane biofilm reactors for autotrophic nitrogen removal: microbial community composition and dynamics.

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