Transcriptome profiling of granulosa cells of bovine ovarian follicles during growth from small to large antral sizes.

ABSTRACT:

Background

At later stages of folliculogenesis, the mammalian ovarian follicle contains layers of epithelial granulosa cells surrounding an antral cavity. During follicle development granulosa cells replicate, secrete hormones and support the growth of the oocyte. In cattle, the follicle needs to grow > 10 mm in diameter to allow an oocyte to ovulate, following which the granulosa cells cease dividing and differentiate into the specialised cells of the corpus luteum. To better understand the molecular basis of follicular growth and granulosa cell maturation, we undertook transcriptome profiling of granulosa cells from small (< 5 mm; n = 10) and large (> 10 mm, n = 4) healthy bovine follicles using Affymetrix microarrays (24,128 probe sets).

Results

Principal component analys

SUBMITTER: Hatzirodos N 

PROVIDER: S-EPMC3898003 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature