Molecular changes in bone marrow, tumor and serum after conductive ablation of murine 4T1 breast carcinoma.

ABSTRACT: Thermal ablation of solid tumors using conductive interstitial thermal therapy (CITT) produces coagulative necrosis in the center of ablation. Local changes in homeostasis for surviving tumor and systemic changes in circulation and distant organs must be understood and monitored in order to prevent tumor re-growth and metastasis. The purpose of this study was to use a mouse carcinoma model to evaluate molecular changes in the bone marrow and surviving tumor after CITT treatment by quantification of transcripts associated with cancer progression and hyperthermia, serum cytokines, stress proteins and the marrow/tumor cross-talk regulator stromal-derived factor 1. Analysis of 27 genes and 22 proteins with quantitative PCR, ELISA, immunoblotting and multiplex antibody assays revealed that the

SUBMITTER: Przybyla BD 

PROVIDER: S-EPMC3898720 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature