Bidirectional promoters are the major source of gene activation-associated non-coding RNAs in mammals.

ABSTRACT:

Background

The majority of non-coding RNAs (ncRNAs) involved in mRNA metabolism in mammals have been believed to downregulate the corresponding mRNA expression level in a pre- or post-transcriptional manner by forming short or long ncRNA-mRNA duplex structures. Information on non-duplex-forming long ncRNAs is now also rapidly accumulating. To examine the directional properties of transcription at the whole-genome level, we performed directional RNA-seq analysis of mouse and chimpanzee tissue samples.

Results

We found that there is only about 1% of the genome where both the top and bottom strands are utilized for transcription, suggesting that RNA-RNA duplexes are not abundantly formed. Focusing on transcription start sites (TSSs) of protein-coding genes revealed that a signi

SUBMITTER: Uesaka M 

PROVIDER: S-EPMC3898825 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature