Characterization of three novel human papillomavirus types isolated from oral rinse samples of healthy individuals.

ABSTRACT:

Background

Despite the strong evidence of HPV infection as the etiological agent in a subset of oral cancer, oral α-HPV detection is rare in healthy individuals, and little is known of the existing of novel HPV types in oral cavity.

Objective

We determined whether novel HPV types can be isolated from oral rinse samples collected from healthy individuals.

Study design

We performed rolling circle amplification (RCA) coupled with degenerated PCR assay on 48 oral rinse samples to amplify novel HPV types. Full length HPV DNA was cloned using long range PCR. Quantitative type specific Taqman assays were used to determine the prevalence of novel HPV types in 158 archived oral tissue samples.

Results

We were able to isolate four novel human papillomavirus types. Full l

SUBMITTER: Martin E 

PROVIDER: S-EPMC3898882 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature