Specific expression of DR5 promoter in rice roots using a tCUP derived promoter-reporter system.

ABSTRACT: Variation of transgene expression caused by either position effect at the insertion site or the promoter/enhancer elements employed for the expression of selectable marker genes has complicated phenotype characterization and caused misinterpretation. We have developed a reporter system in rice to analyze the influence of vector configuration, spacer and selectable marker gene promoter on the expression of the promoterless GUS reporter and DR5 promoter. Our results indicate that a spacer inserted between the reversed 35S promoter and the GUS reporter could reduce leaky expression of the reporter but was unable to block the nonspecific expression of DR5::GUS. Stacking the selectable marker unit in head to tail with the GUS reporter aided the gene specific expression of the GUS reporter under

SUBMITTER: Zhou J 

PROVIDER: S-EPMC3899362 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature