Super-resolution scanning patch clamp reveals clustering of functional ion channels in adult ventricular myocyte.

ABSTRACT:

Rationale

Compartmentation of ion channels on the cardiomyocyte surface is important for electric propagation and electromechanical coupling. The specialized T-tubule and costameric structures facilitate spatial coupling of various ion channels and receptors. Existing methods such as immunofluorescence and patch clamp techniques are limited in their ability to localize functional ion channels. As such, a correlation between channel protein location and channel function remains incomplete.

Objective

To validate a method that permits routine imaging of the topography of a live cardiomyocyte and study clustering of functional ion channels from a specific microdomain.

Methods and results

We used scanning ion conductance microscopy and conventional cell-attached patch clam

SUBMITTER: Bhargava A 

PROVIDER: S-EPMC3899650 | biostudies-literature | 2013 Apr

REPOSITORIES: biostudies-literature