PA28gamma emerges as a novel functional target of tumour suppressor microRNA-7 in non-small-cell lung cancer.

ABSTRACT:

Background

MicroRNA-7 (miR-7) has been reported to be a tumour suppressor gene. However, whether it has a role in the growth of non-small-cell lung cancer (NSCLC) and what is its target involved in the tumour growth is still under investigation.

Methods

NSCLC tissue sample, NSCLC cell lines and tissue microarray were investigated in this study. Total RNA, miRNA and protein were used for RT-PCR and western blot analysis. Immunohistochemistry was performed in tissues microarray. Cell culture and intervention experiments were performed in vitro and in vivo. Bioinformatics prediction, western blot and luciferase assay were identified the target of miR-7.

Results

In this study, we found that the expression of miR-7 was significantly downregulated not only in NSCLC cell lin

SUBMITTER: Xiong S 

PROVIDER: S-EPMC3899764 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature