Targeted transgene integration overcomes variability of position effects in zebrafish.

ABSTRACT: Zebrafish transgenesis is increasingly popular owing to the optical transparency and external development of embryos, which provide a scalable vertebrate model for in vivo experimentation. The ability to express transgenes in a tightly controlled spatio-temporal pattern is an important prerequisite for exploitation of zebrafish in a wide range of biomedical applications. However, conventional transgenesis methods are plagued by position effects: the regulatory environment of genomic integration sites leads to variation of expression patterns of transgenes driven by engineered cis-regulatory modules. This limitation represents a bottleneck when studying the precise function of cis-regulatory modules and their subtle variants or when various effector proteins are to be expressed for labellin

SUBMITTER: Roberts JA 

PROVIDER: S-EPMC3899822 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature