The application of multiple reaction monitoring and multi-analyte profiling to HDL proteins.

ABSTRACT:

Background

HDL carries a rich protein cargo and examining HDL protein composition promises to improve our understanding of its functions. Conventional mass spectrometry methods can be lengthy and difficult to extend to large populations. In addition, without prior enrichment of the sample, the ability of these methods to detect low abundance proteins is limited. Our objective was to develop a high-throughput approach to examine HDL protein composition applicable to diabetes and cardiovascular disease (CVD).

Methods

We optimized two multiplexed assays to examine HDL proteins using a quantitative immunoassay (Multi-Analyte Profiling- MAP) and mass spectrometric-based quantitative proteomics (Multiple Reaction Monitoring-MRM). We screened HDL proteins using human xMAP (90 prote

SUBMITTER: Yassine HN 

PROVIDER: S-EPMC3900256 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature