Genome-wide DNA methylation profiling in cultured eutopic and ectopic endometrial stromal cells.

ABSTRACT: The objective of this study was to characterize the genome-wide DNA methylation profiles of isolated endometrial stromal cells obtained from eutopic endometria with (euESCa) and without endometriosis (euESCb) and ovarian endometrial cysts (choESC). Three samples were analyzed in each group. The infinium methylation array identified more hypermethylated and hypomethylated CpGs in choESC than in euESCa, and only a few genes were methylated differently in euESCa and euESCb. A functional analysis revealed that signal transduction, developmental processes, immunity, etc. were different in choESC and euESCa. A clustering analysis and a principal component analysis performed based on the methylation levels segregated choESC from euESC, while euESCa and euESCb were identical. A transcriptome analy

SUBMITTER: Yamagata Y 

PROVIDER: S-EPMC3900404 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature