Genome-wide transcriptome and antioxidant analyses on gamma-irradiated phases of deinococcus radiodurans R1.

ABSTRACT: Adaptation of D. radiodurans cells to extreme irradiation environments requires dynamic interactions between gene expression and metabolic regulatory networks, but studies typically address only a single layer of regulation during the recovery period after irradiation. Dynamic transcriptome analysis of D. radiodurans cells using strand-specific RNA sequencing (ssRNA-seq), combined with LC-MS based metabolite analysis, allowed an estimate of the immediate expression pattern of genes and antioxidants in response to irradiation. Transcriptome dynamics were examined in cells by ssRNA-seq covering its predicted genes. Of the 144 non-coding RNAs that were annotated, 49 of these were transfer RNAs and 95 were putative novel antisense RNAs. Genes differentially expressed during irradiation and rec

SUBMITTER: Luan H 

PROVIDER: S-EPMC3900439 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature