Efficient yeast cell-surface display of exo- and endo-cellulase using the SED1 anchoring region and its original promoter.

ABSTRACT:

Background

The recombinant yeast strains displaying the heterologous cellulolytic enzymes on the cell surface using the glycosylphosphatidylinositol (GPI) anchoring system are considered promising biocatalysts for direct conversion of lignocellulosic materials to ethanol. However, the cellulolytic activities of the conventional cellulase-displaying yeast strains are insufficient for the hydrolysis of cellulose. In this study, we constructed novel gene cassettes for the efficient cellulose utilization by cellulase-displaying yeast strains.

Results

The novel gene cassettes for the cell-surface display of Aspergillus aculeatus β-glucosidase (BGL1) and Trichoderma reeseii endoglucanase II (EGII) were constructed using the promoter and the GPI anchoring region derived from Saccha

SUBMITTER: Inokuma K 

PROVIDER: S-EPMC3900695 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature