Distinct DNA-based epigenetic switches trigger transcriptional activation of silent genes in human dermal fibroblasts.

ABSTRACT: The influential role of the epigenome in orchestrating genome-wide transcriptional activation instigates the demand for the artificial genetic switches with distinct DNA sequence recognition. Recently, we developed a novel class of epigenetically active small molecules called SAHA-PIPs by conjugating selective DNA binding pyrrole-imidazole polyamides (PIPs) with the histone deacetylase inhibitor SAHA. Screening studies revealed that certain SAHA-PIPs trigger targeted transcriptional activation of pluripotency and germ cell genes in mouse and human fibroblasts, respectively. Through microarray studies and functional analysis, here we demonstrate for the first time the remarkable ability of thirty-two different SAHA-PIPs to trigger the transcriptional activation of exclusive clusters of gene

SUBMITTER: Pandian GN 

PROVIDER: S-EPMC3900999 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature