Disarming Burkholderia pseudomallei: structural and functional characterization of a disulfide oxidoreductase (DsbA) required for virulence in vivo.

ABSTRACT:

Aims

The intracellular pathogen Burkholderia pseudomallei causes the disease melioidosis, a major source of morbidity and mortality in southeast Asia and northern Australia. The need to develop novel antimicrobials is compounded by the absence of a licensed vaccine and the bacterium's resistance to multiple antibiotics. In a number of clinically relevant Gram-negative pathogens, DsbA is the primary disulfide oxidoreductase responsible for catalyzing the formation of disulfide bonds in secreted and membrane-associated proteins. In this study, a putative B. pseudomallei dsbA gene was evaluated functionally and structurally and its contribution to infection assessed.

Results

Biochemical studies confirmed the dsbA gene encodes a protein disulfide oxidoreductase. A dsbA deletion

SUBMITTER: Ireland PM 

PROVIDER: S-EPMC3901323 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature