An integrated micro-electro-fluidic and protein arraying system for parallel analysis of cell responses to controlled microenvironments.

ABSTRACT: Living cells have evolved sophisticated signaling networks allowing them to respond to a wide array of external stimuli. Microfluidic devices, facilitating the analysis of signaling networks through precise definition of the cellular microenvironment often lack the capacity of delivering multiple combinations of different signaling cues, thus limiting the throughput of the analysis. To address this limitation, we developed a microfabricated platform combining microfluidic definition of the cell medium composition with dielectrophoretic definition of cell positions and protein microarray-based presentation of diverse signaling inputs. Ligands combined at different concentrations were spotted along with an extracellular matrix protein onto a glass substratum in alignment with an electrode ar

SUBMITTER: Yin Z 

PROVIDER: S-EPMC3902009 | biostudies-literature | 2010 Sep

REPOSITORIES: biostudies-literature