Small molecule-mediated directed differentiation of human embryonic stem cells toward ventricular cardiomyocytes.

ABSTRACT: The generation of human ventricular cardiomyocytes from human embryonic stem cells and/or induced pluripotent stem cells could fulfill the demand for therapeutic applications and in vitro pharmacological research; however, the production of a homogeneous population of ventricular cardiomyocytes remains a major limitation. By combining small molecules and growth factors, we developed a fully chemically defined, directed differentiation system to generate ventricular-like cardiomyocytes (VCMs) from human embryonic stem cells and induced pluripotent stem cells with high efficiency and reproducibility. Molecular characterization revealed that the differentiation recapitulated the developmental steps of cardiovascular fate specification. Electrophysiological analyses further illustrated the gen

SUBMITTER: Karakikes I 

PROVIDER: S-EPMC3902291 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature