Intensity-based analysis of two-colour microarrays enables efficient and flexible hybridization designs.

ABSTRACT: In two-colour microarrays, the ratio of signal intensities of two co-hybridized samples is used as a relative measure of gene expression. Ratio-based analysis becomes complicated and inefficient in multi-class comparisons. We therefore investigated the validity of an intensity-based analysis procedure. To this end, two different cRNA targets were hybridized together, separately, with a common reference and in a self-self fashion on spotted 65mer oligonucleotide microarrays. We found that the signal intensity of the cRNA targets was not influenced by the presence of a target labelled in the opposite colour. This indicates that targets do not compete for binding sites on the array, which is essential for intensity-based analysis. It is demonstrated that, for good-quality arrays, the correlat

SUBMITTER: 't Hoen PA 

PROVIDER: S-EPMC390313 | biostudies-literature | 2004 Feb

REPOSITORIES: biostudies-literature