Cohesin and CTCF differentially affect chromatin architecture and gene expression in human cells.

ABSTRACT: Recent studies of genome-wide chromatin interactions have revealed that the human genome is partitioned into many self-associating topological domains. The boundary sequences between domains are enriched for binding sites of CTCC-binding factor (CTCF) and the cohesin complex, implicating these two factors in the establishment or maintenance of topological domains. To determine the role of cohesin and CTCF in higher-order chromatin architecture in human cells, we depleted the cohesin complex or CTCF and examined the consequences of loss of these factors on higher-order chromatin organization, as well as the transcriptome. We observed a general loss of local chromatin interactions upon disruption of cohesin, but the topological domains remain intact. However, we found that depletion of CTCF

SUBMITTER: Zuin J 

PROVIDER: S-EPMC3903193 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature