Cyanobacterial aldehyde deformylase oxygenation of aldehydes yields n-1 aldehydes and alcohols in addition to alkanes.

ABSTRACT: Aldehyde-deformylating oxygenase (ADO) catalyzes O₂-dependent release of the terminal carbon of a biological substrate, octadecanal, to yield formate and heptadecane in a reaction that requires external reducing equivalents. We show here that ADO also catalyzes incorporation of an oxygen atom from O₂ into the alkane product to yield alcohol and aldehyde products. Oxygenation of the alkane product is much more pronounced with C_9-10 aldehyde substrates, so that use of nonanal as the substrate yields similar amounts of octane, octanal, and octanol products. When using doubly-labeled [1,2-¹³C]-octanal as the substrate, the heptane, heptanal and heptanol products each contained a single ¹³C-label in the C-1 carbons atoms. The only one-carbo

SUBMITTER: Aukema KG 

PROVIDER: S-EPMC3903409 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature