The HOG pathway is critical for the colonization of the mouse gastrointestinal tract by Candida albicans.

ABSTRACT: The opportunistic pathogen Candida albicans is a frequent inhabitant of the human gastrointestinal tract where it usually behaves as a harmless commensal. In this particular niche, it needs to adapt to the different micro environments that challenge its survival within the host. In order to determine those factors involved in gut adaptation, we have used a gastrointestinal model of colonization in mouse to trace the behaviour of fungal cells. We have developed a genetic labelling system based on the complementary spectral properties of the fluorescent proteins GFP and a new C. albicans codon-adapted RFP (dTOM2) that allow a precise quantification of the fungal population in the gut via standard in vitro cultures or flow cytometry. This methodology has allowed us to determine the role of th

SUBMITTER: Prieto D 

PROVIDER: S-EPMC3903619 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature