Reporters transiently transfected into mammalian cells are highly sensitive to translational repression induced by dsRNA expression.

ABSTRACT: In mammals, double-stranded RNA (dsRNA) can mediate sequence-specific RNA interference, activate sequence-independent interferon response, or undergo RNA editing by adenosine deaminases. We showed that long hairpin dsRNA expression had negligible effects on mammalian somatic cells--expressed dsRNA was slightly edited, poorly processed into siRNAs, and it did not activate the interferon response. At the same time, we noticed reduced reporter expression in transient co-transfections, which was presumably induced by expressed dsRNA. Since transient co-transfections are frequently used for studying gene function, we systematically explored the role of expressed dsRNA in this silencing phenomenon. We demonstrate that dsRNA expressed from transiently transfected plasmids strongly inhibits the ex

SUBMITTER: Nejepinska J 

PROVIDER: S-EPMC3903663 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature