Genetic programs constructed from layered logic gates in single cells.

ABSTRACT: Genetic programs function to integrate environmental sensors, implement signal processing algorithms and control expression dynamics. These programs consist of integrated genetic circuits that individually implement operations ranging from digital logic to dynamic circuits, and they have been used in various cellular engineering applications, including the implementation of process control in metabolic networks and the coordination of spatial differentiation in artificial tissues. A key limitation is that the circuits are based on biochemical interactions occurring in the confined volume of the cell, so the size of programs has been limited to a few circuits. Here we apply part mining and directed evolution to build a set of transcriptional AND gates in Escherichia coli. Each AND gate inte

SUBMITTER: Moon TS 

PROVIDER: S-EPMC3904217 | biostudies-literature | 2012 Nov

REPOSITORIES: biostudies-literature