Ultrasensitive and fast bottom-up analysis of femtogram amounts of complex proteome digests.

ABSTRACT: Femtogram proteomics: An ultrasensitive capillary zone electrophoresis-mass spectrometry system that is based on an improved nanospray interface has been developed. This system is used for the analysis of picogram to femtogram amounts of E. coli digests; for example, over 100 proteins were identified from 16 pg digests by tandem mass spectrometry. AMTs=accurate mass and time tags.

SUBMITTER: Sun L 

PROVIDER: S-EPMC3904452 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature