Deep imaging: how much of the proteome does current top-down technology already resolve?

ABSTRACT: Effective proteome analyses are based on interplay between resolution and detection. It had been claimed that resolution was the main factor limiting the use of two-dimensional gel electrophoresis. Improved protein detection now indicates that this is unlikely to be the case. Using a highly refined protocol, the rat brain proteome was extracted, resolved, and detected. In order to overcome the stain saturation threshold, high abundance protein species were excised from the gel following standard imaging. Gels were then imaged again using longer exposure times, enabling detection of lower abundance, less intensely stained protein species. This resulted in a significant enhancement in the detection of resolved proteins, and a slightly modified digestion protocol enabled effective identificat

SUBMITTER: Wright EP 

PROVIDER: S-EPMC3904854 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature