Imaging of molecular surface dynamics in brain slices using single-particle tracking.

ABSTRACT: Organization of signalling molecules in biological membranes is crucial for cellular communication. Many receptors, ion channels and cell adhesion molecules are associated with proteins important for their trafficking, surface localization or function. These complexes are embedded in a lipid environment of varying composition. Binding affinities and stoichiometry of such complexes were so far experimentally accessible only in isolated systems or monolayers of cell culture. Visualization of molecular dynamics within signalling complexes and their correlation to specialized membrane compartments demand high temporal and spatial resolution and has been difficult to demonstrate in complex tissue like brain slices. Here we demonstrate the feasibility of single-particle tracking (SPT) in organot

SUBMITTER: Biermann B 

PROVIDER: S-EPMC3905702 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature