Histone posttranslational modifications and cell fate determination: lens induction requires the lysine acetyltransferases CBP and p300.

ABSTRACT: Lens induction is a classical embryologic model to study cell fate determination. It has been proposed earlier that specific changes in core histone modifications accompany the process of cell fate specification and determination. The lysine acetyltransferases CBP and p300 function as principal enzymes that modify core histones to facilitate specific gene expression. Herein, we performed conditional inactivation of both CBP and p300 in the ectodermal cells that give rise to the lens placode. Inactivation of both CBP and p300 resulted in the dramatic discontinuation of all aspects of lens specification and organogenesis, resulting in aphakia. The CBP/p300(-/-) ectodermal cells are viable and not prone to apoptosis. These cells showed reduced expression of Six3 and Sox2, while expression of

SUBMITTER: Wolf L 

PROVIDER: S-EPMC3905850 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature