Mismatch repair protein MSH2 regulates translesion DNA synthesis following exposure of cells to UV radiation.

ABSTRACT: Translesion DNA synthesis (TLS) can use specialized DNA polymerases to insert and/or extend nucleotides across lesions, thereby limiting stalled replication fork collapse and the potential for cell death. Recent studies have shown that monoubiquitinated proliferating cell nuclear antigen (PCNA) plays an important role in recruitment of Y-family TLS polymerases to stalled replication forks after DNA damage treatment. To explore the possible roles of other factors that regulate the ultraviolet (UV)-induced assembly of specialized DNA polymerases at arrested replication forks, we performed immunoprecipitation experiments combined with mass spectrometry and established that DNA polymerase kappa (Polκ) can partner with MSH2, an important mismatch repair protein associated with hereditary non-po

SUBMITTER: Lv L 

PROVIDER: S-EPMC3905884 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature