Highly efficient selection of epitope specific antibody through competitive yeast display library sorting.

ABSTRACT: Combinatory antibody library display technologies have been invented and successfully implemented for the selection and engineering of therapeutic antibodies. Precise targeting of important epitopes on the protein of interest is essential for such isolated antibodies to serve as effective modulators of molecular interactions. We developed a strategy to efficiently isolate antibodies against a specific epitope on a target protein from a yeast display antibody library using dengue virus envelope protein domain III as a model target. A domain III mutant protein with a key mutation inside a cross-reactive neutralizing epitope was designed, expressed, and used in the competitive panning of a yeast display naïve antibody library. All the yeast display antibodies that bound to the wild type domai

SUBMITTER: Puri V 

PROVIDER: S-EPMC3906307 | biostudies-literature | 2013 Jul-Aug

REPOSITORIES: biostudies-literature