Oxidative stress induced by HIV-1 reverse transcriptase modulates the enzyme's performance in gene immunization.

ABSTRACT: HIV-1 infection induces chronic oxidative stress. The resultant neurotoxicity has been associated with Tat protein. Here, we for the first time describe the induction of oxidative stress by another HIV-1 protein, reverse transcriptase (RT). Expression of HIV-1 RT in human embryonic kidney cells generated potent production of the reactive oxygen species (ROS), detected by the fluorescence-based probes. Quantitative RT-PCR demonstrated that expression of RT in HEK293 cells induced a 10- to 15-fold increased transcription of the phase II detoxifying enzymes human

Nad(p)h

quinone oxidoreductase (Nqo1) and heme oxygenase 1 (HO-1), indicating the induction of oxidative stress response. The capacity to induce oxidative stress and stress response appeared to be an intrinsic property of a va

SUBMITTER: Isaguliants M 

PROVIDER: S-EPMC3906395 | biostudies-literature | 2013 Oct

REPOSITORIES: biostudies-literature