The human hepatic cell line HepaRG as a possible cell source for the generation of humanized liver TK-NOG mice.

ABSTRACT: 1. Humanized-liver mice, in which the liver has been repopulated with human hepatocytes, have been used to study aspects of human liver physiology such as drug metabolism, toxicology and hepatitis infection. However, the procurement of human hepatocytes is a major problem in producing humanized-liver mice because of the finite nature of the patient-derived resource. 2. In order to overcome this limitation, the human hepatic cell line HepaRG® were evaluated as promising donor cells for liver reconstitution in the TK-NOG mouse model. 3. We demonstrate that, in vivo, transplanted confluent culture or differentiated HepaRG® cells proliferated and differentiated toward both hepatocyte-like and biliary-like cells within the recipient liver. In contrast, proliferative HepaRG® cells could engraft

SUBMITTER: Higuchi Y 

PROVIDER: S-EPMC3906414 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature