Sequence-based separation of single-stranded DNA using nucleotides in capillary electrophoresis: focus on phosphate.

ABSTRACT: DNA analysis has widespread applicability in biology, medicine, biotechnology, and forensics. DNA separation by length is readily achieved using sieving gels in electrophoresis. Separation by sequence is less simple, generally requiring adequate differences in native or induced conformation or differences in thermal or chemical stability of the strands that are hybridized prior to measurement. We previously demonstrated separation of four single-stranded DNA 76-mers that differ by only a few A-G substitutions based solely on sequence using guanosine-5'-monophosphate (GMP) in the running buffer. We attributed separation to the unique self-assembly of GMP to form higher order structures. Here, we examine an expanded set of 76-mers designed to probe the mechanism of the separation and effects

SUBMITTER: Zhang X 

PROVIDER: S-EPMC3906628 | biostudies-literature | 2013 Jun

REPOSITORIES: biostudies-literature