A genome-wide survey of maternal and embryonic transcripts during Xenopus tropicalis development.

ABSTRACT:

Background

Dynamics of polyadenylation vs. deadenylation determine the fate of several developmentally regulated genes. Decay of a subset of maternal mRNAs and new transcription define the maternal-to-zygotic transition, but the full complement of polyadenylated and deadenylated coding and non-coding transcripts has not yet been assessed in Xenopus embryos.

Results

To analyze the dynamics and diversity of coding and non-coding transcripts during development, both polyadenylated mRNA and ribosomal RNA-depleted total RNA were harvested across six developmental stages and subjected to high throughput sequencing. The maternally loaded transcriptome is highly diverse and consists of both polyadenylated and deadenylated transcripts. Many maternal genes show peak expression in the

SUBMITTER: Paranjpe SS 

PROVIDER: S-EPMC3907017 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature