Using phage display selected antibodies to dissect microbiomes for complete de novo genome sequencing of low abundance microbes.

ABSTRACT:

Background

Single cell genomics has revolutionized microbial sequencing, but complete coverage of genomes in complex microbiomes is imperfect due to enormous variation in organismal abundance and amplification bias. Empirical methods that complement rapidly improving bioinformatic tools will improve characterization of microbiomes and facilitate better genome coverage for low abundance microbes.

Methods

We describe a new approach to sequencing individual species from microbiomes that combines antibody phage display against intact bacteria with fluorescence activated cell sorting (FACS). Single chain (scFv) antibodies are selected using phage display against a bacteria or microbial community, resulting in species-specific antibodies that can be used in FACS for relative quant

SUBMITTER: Close DW 

PROVIDER: S-EPMC3907030 | biostudies-literature | 2013 Nov

REPOSITORIES: biostudies-literature