Growth cone-specific functions of XMAP215 in restricting microtubule dynamics and promoting axonal outgrowth.

ABSTRACT:

Background

Microtubule (MT) regulators play essential roles in multiple aspects of neural development. In vitro reconstitution assays have established that the XMAP215/Dis1/TOG family of MT regulators function as MT 'plus-end-tracking proteins' (+TIPs) that act as processive polymerases to drive MT growth in all eukaryotes, but few studies have examined their functions in vivo. In this study, we use quantitative analysis of high-resolution live imaging to examine the function of XMAP215 in embryonic Xenopus laevis neurons.

Results

Here, we show that XMAP215 is required for persistent axon outgrowth in vivo and ex vivo by preventing actomyosin-mediated axon retraction. Moreover, we discover that the effect of XMAP215 function on MT behavior depends on cell type and context. W

SUBMITTER: Lowery LA 

PROVIDER: S-EPMC3907036 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature