Generation of a human airway epithelium derived basal cell line with multipotent differentiation capacity.

ABSTRACT:

Background

As the multipotent progenitor population of the airway epithelium, human airway basal cells (BC) replenish the specialized differentiated cell populations of the mucociliated airway epithelium during physiological turnover and repair. Cultured primary BC divide a limited number of times before entering a state of replicative senescence, preventing the establishment of long-term replicating cultures of airway BC that maintain their original phenotype.

Methods

To generate an immortalized human airway BC cell line, primary human airway BC obtained by brushing the airway epithelium of healthy nonsmokers were infected with a retrovirus expressing human telomerase (hTERT). The resulting immortalized cell line was then characterized under non-differentiating and differen

SUBMITTER: Walters MS 

PROVIDER: S-EPMC3907041 | biostudies-literature | 2013 Dec

REPOSITORIES: biostudies-literature