Selection and validation of reference genes for quantitative gene expression studies in Erythroxylum coca.

ABSTRACT: Real-time quantitative PCR is a powerful technique for the investigation of comparative gene expression, but its accuracy and reliability depend on the reference genes used as internal standards. Only genes that show a high level of expression stability are suitable for use as reference genes, and these must be identified on a case-by-case basis. Erythroxylum coca produces and accumulates high amounts of the pharmacologically active tropane alkaloid cocaine (especially in the leaves), and is an emerging model for the investigation of tropane alkaloid biosynthesis. The identification of stable internal reference genes for this species is important for its development as a model species, and would enable comparative analysis of candidate biosynthetic genes in the different tissues of the coc

SUBMITTER: Docimo T 

PROVIDER: S-EPMC3907153 | biostudies-literature | 2013

REPOSITORIES: biostudies-literature