Variation in genome-wide levels of meiotic recombination is established at the onset of prophase in mammalian males.

ABSTRACT: Segregation of chromosomes during the first meiotic division relies on crossovers established during prophase. Although crossovers are strictly regulated so that at least one occurs per chromosome, individual variation in crossover levels is not uncommon. In an analysis of different inbred strains of male mice, we identified among-strain variation in the number of foci for the crossover-associated protein MLH1. We report studies of strains with "low" (CAST/EiJ), "medium" (C3H/HeJ), and "high" (C57BL/6J) genome-wide MLH1 values to define factors responsible for this variation. We utilized immunofluorescence to analyze the number and distribution of proteins that function at different stages in the recombination pathway: RAD51 and DMC1, strand invasion proteins acting shortly after double-st

SUBMITTER: Baier B 

PROVIDER: S-EPMC3907295 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature