Reciprocal upregulation of Notch signaling molecules in hematopoietic progenitor and mesenchymal stromal cells.

ABSTRACT: Although mesenchymal stem cells (MSCs) play pivotal supportive roles in hematopoiesis, how they interact with hematopoietic stem cells (HSCs) is not well understood. We investigated the interaction between HSCs and surrogate MSCs (C3H10T1/2 stromal cells), focusing on the molecular events induced by cell contact of these bipartite populations. C3H10T1/2 is a mesenchymal stromal cell line that can be induced to differentiate into preadipocytes (A54) and myoblasts (M1601). The stromal cell derivatives were cocultured with murine HSCs (Lineage(-)Sca1(+)), and gene expression profiles in stromal cells and HSCs were compared before and after the coculture. HSCs gave rise to cobblestone areas only on A54 cells, with ninefold more progenitors than on M1601 or undifferentiated C3H10T1/2 cells. Mic

SUBMITTER: Kikuchi Y 

PROVIDER: S-EPMC3908275 | biostudies-literature | 2011

REPOSITORIES: biostudies-literature