A robotic MCF-7:WS8 cell proliferation assay to detect agonist and antagonist estrogenic activity.

ABSTRACT: Endocrine-disrupting chemicals with estrogenic activity (EA) or anti-EA (AEA) have been extensively reported to possibly have many adverse health effects. We have developed robotized assays using MCF-7:WS8 cell proliferation (or suppression) to detect EA (or AEA) of 78 test substances supplied by the Interagency Coordinating Committee on the Validation of Alternative Methods and the National Toxicology Program's Interagency Center for the Evaluation of Alternative Toxicological Methods for validation studies. We also assayed ICI 182,780, a strong estrogen antagonist. Chemicals to be assayed were initially examined for solubility and volatility to determine optimal assay conditions. For both EA and AEA determinations, a Range-Finder assay was conducted to determine the concentration range f

SUBMITTER: Yang CZ 

PROVIDER: S-EPMC3908721 | biostudies-literature | 2014 Feb

REPOSITORIES: biostudies-literature