Block copolymer/DNA vaccination induces a strong allergen-specific local response in a mouse model of house dust mite asthma.

ABSTRACT:

Background

Allergic asthma is caused by abnormal immunoreactivity against allergens such as house dust mites among which Dermatophagoides farinae (Der f) is a common species. Currently, immunotherapy is based on allergen administration, which has variable effect from patient to patient and may cause serious side effects, principally the sustained risk of anaphylaxis. DNA vaccination is a promising approach by triggering a specific immune response with reduced allergenicity.

Objective

The aim of the study is to evaluate the effects of DNA immunization with Der f1 allergen specific DNA on allergic sensitization, inflammation and respiratory function in mice.

Methods

Mice were vaccinated 28 and 7 days before allergen exposure with a Der f1-encoding plasmid formulated wit

SUBMITTER: Rolland-Debord C 

PROVIDER: S-EPMC3908923 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature