Identification of the maize gravitropism gene lazy plant1 by a transposon-tagging genome resequencing strategy.

ABSTRACT: Since their initial discovery, transposons have been widely used as mutagens for forward and reverse genetic screens in a range of organisms. The problems of high copy number and sequence divergence among related transposons have often limited the efficiency at which tagged genes can be identified. A method was developed to identity the locations of Mutator (Mu) transposons in the Zea mays genome using a simple enrichment method combined with genome resequencing to identify transposon junction fragments. The sequencing library was prepared from genomic DNA by digesting with a restriction enzyme that cuts within a perfectly conserved motif of the Mu terminal inverted repeats (TIR). Paired-end reads containing Mu TIR sequences were computationally identified and chromosomal sequences flankin

SUBMITTER: Howard TP 

PROVIDER: S-EPMC3909067 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature