Differences in cumulus cells gene expression between modified natural and stimulated in vitro fertilization cycles.

ABSTRACT:

Purpose

The aim of our study was to determine whether there are any differences in the cumulus cell gene expression profile of mature oocytes derived from modified natural IVF and controlled ovarian hyperstimulation cycles and if these changes could help us understand why modified natural IVF has lower success rates.

Methods

Cumulus cells surrounding mature oocytes that developed to morulae or blastocysts on day 5 after oocyte retrieval were submitted to microarray analysis. The obtained data were then validated using quantitative real-time PCR.

Results

There were 66 differentially expressed genes between cumulus cells of modified natural IVF and controlled ovarian hyperstimulation cycles. Gene ontology analysis revealed the oxidation-reduction process, glutathione me

SUBMITTER: Papler TB 

PROVIDER: S-EPMC3909132 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature