MicroRNA-10a is down-regulated by DNA methylation and functions as a tumor suppressor in gastric cancer cells.

ABSTRACT:

Background

MicroRNAs act as posttranscriptional regulators of gene expression in many biological processes. Their deregulations occur commonly in gastric cancer (GC). Although DNA methylation constitutes an important mechanism for microRNA deregulation in cancer, this field largely remains unexplored.

Methodology/principal findings

Total RNA was extracted from the tissues of 100 patients with GC and four gastric cancer cell lines. The expression levels of miR-10a were determined by real-time PCR with specific TaqMan probes. Moreover, a functional analysis of miR-10a in regulating cell proliferation, migration and invasion was performed. Subsequently, quantitative methylation-specific PCR (qMSP) was used to detect the DNA methylation status in the CpG islands upstream of miR-

SUBMITTER: Jia H 

PROVIDER: S-EPMC3909310 | biostudies-literature | 2014

REPOSITORIES: biostudies-literature