Drug-resistance associated mutations in polymerase (p) gene of hepatitis B virus isolated from malaysian HBV carriers.

ABSTRACT:

Background

Mutations in the polymerase (P) gene of hepatitis B virus are often associated with drug resistance. The pattern of mutations varies geographically, thus giving rise to genotypes diversity.

Objectives

This study was carried out to detect mutations in P gene of hepatitis B virus isolated from Malaysian HBV carriers.

Materials and methods

A total of 58 sera samples were analyzed by PCR and sequencing, of which the P gene of isolated HBV was successfully amplified and sequenced from 40 samples.

Results

Genotyping of these samples revealed that the predominant genotype was genotype C (22/40, 55.0%), followed by genotype B (17/40, 42.5%), and only 1 sample showed genotype D (2.5%). A number of significant drug resistant mutations were found in five patien

SUBMITTER: Suppiah J 

PROVIDER: S-EPMC3909636 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature