Ontology highlight
ABSTRACT:
SUBMITTER: Rouhana L
PROVIDER: S-EPMC3909682 | biostudies-literature | 2013 Jun
REPOSITORIES: biostudies-literature
Rouhana Labib L Weiss Jennifer A JA Forsthoefel David J DJ Lee Hayoung H King Ryan S RS Inoue Takeshi T Shibata Norito N Agata Kiyokazu K Newmark Phillip A PA
Developmental dynamics : an official publication of the American Association of Anatomists 20130401 6
<h4>Background</h4>The ability to assess gene function is essential for understanding biological processes. Currently, RNA interference (RNAi) is the only technique available to assess gene function in planarians, in which it has been induced by means of injection of double-stranded RNA (dsRNA), soaking, or ingestion of bacteria expressing dsRNA.<h4>Results</h4>We describe a simple and robust RNAi protocol, involving in vitro synthesis of dsRNA that is fed to the planarians. Advantages of this p ...[more]