Presence of acyl-homoserine lactones in 57 members of the Vibrionaceae family.

ABSTRACT:

Aims

The aim of this study was to use a sensitive method to screen and quantify 57 Vibrionaceae strains for the production of acyl-homoserine lactones (AHLs) and map the resulting AHL profiles onto a host phylogeny.

Methods and results

We used a high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) protocol to measure AHLs in spent media after bacterial growth. First, the presence/absence of AHLs (qualitative analysis) was measured to choose internal standard for subsequent quantitative AHL measurements. We screened 57 strains from three genera (Aliivibrio, Photobacterium and Vibrio) of the same family (i.e. Vibrionaceae). Our results show that about half of the isolates produced multiple AHLs, typically at 25-5000 nmol l(-1) .

Conclusions

This

SUBMITTER: Purohit AA 

PROVIDER: S-EPMC3910146 | biostudies-literature | 2013 Sep

REPOSITORIES: biostudies-literature