MiRNA-34b inhibits prostate cancer through demethylation, active chromatin modifications, and AKT pathways.

ABSTRACT:

Purpose

miRNAs can act as oncomirs or tumor-suppressor miRs in cancer. This study was undertaken to investigate the status and role of miR-34b in prostate cancer.

Experimental design

Profiling of miR-34b was carried out in human prostate cancer cell lines and clinical samples by quantitative real-time PCR and in situ hybridization. Statistical analyses were done to assess diagnostic/prognostic potential. Biological significance was elucidated by carrying out a series of experiments in vitro and in vivo.

Results

We report that miR-34b is silenced in human prostate cancer and the mechanism is through CpG hypermethylation. miR-34b directly targeted methyltransferases and deacetylases resulting in a positive feedback loop inducing partial demethylation and active chromati

SUBMITTER: Majid S 

PROVIDER: S-EPMC3910324 | biostudies-literature | 2013 Jan

REPOSITORIES: biostudies-literature