Expression and activity of collagenases in the digital laminae of horses with carbohydrate overload-induced acute laminitis.

ABSTRACT:

Background

Matrix metalloproteinases (MMP) are hypothesized to degrade structurally important components of the laminar extracellular matrix (ECM) in horses with laminitis.

Objective

To compare levels of expression of stromelysin-1 (MMP-3), collagenases (MMP-1, -13), and membrane type-MMPs (MMP-14, -15, -16), and the distribution of their ECM substrates, in laminae of healthy horses and horses with carbohydrate overload laminitis.

Animals

Twenty-five adult horses.

Methods

Gene and protein expression were determined in extracts of laminae using real-time quantitative polymerase chain reaction and Western blotting after sodium dodecylsulfate polyacrylamide gel electrophoresis. Distribution of MMP-13 and ECM components was determined using indirect immunofluoresce

SUBMITTER: Wang L 

PROVIDER: S-EPMC3910380 | biostudies-literature | 2014 Jan-Feb

REPOSITORIES: biostudies-literature