Autodisplay for the co-expression of lipase and foldase on the surface of E. coli: washing with designer bugs.

ABSTRACT:

Background

Lipases including the lipase from Burkholderia cepacia are in a main focus in biotechnology research since many years because of their manifold possibilities for application in industrial processes. The application of Burkholderia cepacia lipase for these processes appears complicated because of the need for support by a chaperone, the lipase specific foldase. Purification and reconstitution protocols therefore interfere with an economic implementation of such enzymes in industry. Autodisplay is a convenient method to express a variety of passenger proteins on the surface of E. coli. This method makes subsequent purification steps to obtain the protein of interest unnecessary. If enzymes are used as passengers, the corresponding cells can simply be applied as whole cell

SUBMITTER: Kranen E 

PROVIDER: S-EPMC3910678 | biostudies-literature | 2014 Jan

REPOSITORIES: biostudies-literature